BrightLine® image-splitting dichroic beamsplitters offer superb image quality for both transmitted and reflected light when separating beams of light by color for simultaneous capture of multi-color images. For applications such as (FRET) and real-time live-cell imaging, users can now separate two, four or even more colors onto as many cameras or regions of a single camera sensor. The exceptional flatness of these filters virtually eliminates aberrations in the reflected beam for most common imaging systems. This dichroic beamsplitter is optimized for reflecting imaging beams up to 10 mm in diameter while minimizing RWE.
Common Fluorophore Pairs to Split: GFP/mCherry or FITC/TxRed
Interested in an improved Flatness / RWE version? Consider Semrock’s image-splitting dichoric beamsplitter for super-resolution microscopy:
λ/5 P-V RWE on 3mm FF580-FDi02-t3, optimized for reflecting imaging beams up to 37 mm in custom size versions.
25.2 mm x 35.6 mm x 1.1 mm
low-autofluorescence optical quality glass
Ravg > 95% 350 – 570 nm
Tavg > 93% 590.8 – 950 nm
45 ± 1.5 degrees
Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hrs.
25.2 mm x 35.6 mm
± 0.1 mm
± 0.05 mm
≥ 80% (elliptical)
Reflective surface marked with part number - Orient in direction of incoming light